human skin fibroblasts hs68  (ATCC)

Journal: Biomolecules

Article Title: Improvement of Skin Condition Through RXR Alpha-Activating Materials

doi: 10.3390/biom15020296

Figure Lengend Snippet: Effect of andrographolide and BPE on collagen synthesis in skin cells. ( a ) Protein level of pro-collagen type 1 alpha 1 secreted by skin cells (Hs68) was enhanced by combined treatment of andrographolide and BPE ( n = 6 per group). ( b ) Protein level of MMP-1 secreted by skin cells (Hs68) was reduced by combined treatment of andrographolide and BPE ( n = 4–5). ( c ) Gene expressions of different types of collagens were enhanced by co-treatment of andrographolide and BPE ( n = 6 per group). ( d ) Representative images of artificial 3D skin. Scale bar = 275 μm. The blue color represents collagen. ( e ) Relative collagen content in dermal area of artificial 3D skin. Collagen content was enhanced by combined treatment of andrographolide and BPE ( n = 5–6) Error bars represent standard error of the mean. * p < 0.05, *** p < 0.001; one-way ANOVA analysis, Student’s t -test and Kruskal–Wallis test for nonparametric statistics.

Article Snippet: Human skin keratinocytes (HaCaT) and human skin fibroblasts (Hs68) were purchased from AddexBio (T0020001, San Diego, CA, USA) and the American Type Culture Collection (CRL-1635, Manassas, VA, USA), respectively.

Techniques:

Journal: Biomolecules

Article Title: Improvement of Skin Condition Through RXR Alpha-Activating Materials

doi: 10.3390/biom15020296

Figure Lengend Snippet: Effect of the combined treatment of andrographolide and BPE on ECM components. ( a ) Relative expression of ECM-related genes in Hs68 treated with active materials. ELN , FBN1 and FN1 gene expressions were enhanced by combined treatment of andrographolide and BPE ( n = 6 per group). ( b ) Representative images of immunofluorescence staining for fibrillin-1 (Green). Nuclei were stained with DAPI. Scale bar = 125 μm. ( c ) Relative fluorescence intensity normalized to DAPI. Fibrillin-1 fluorescence intensity was enhanced by combined treatment of andrographolide and BPE ( n = 5–8). Error bars indicate standard error of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001; one-way ANOVA analysis and Student’s t -test.

Article Snippet: Human skin keratinocytes (HaCaT) and human skin fibroblasts (Hs68) were purchased from AddexBio (T0020001, San Diego, CA, USA) and the American Type Culture Collection (CRL-1635, Manassas, VA, USA), respectively.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Effects of triterpenes (10 μM) on cell viability ( A ), protective effect ( B ), UVB-induced reactive oxygen species production ( C ), GSH depletion ( D ), and MDA production ( E ) in Hs68 cells. Values are expressed as means ± SD ( n = 3). Different letters indicate significant ( p < 0.05) differences based on Duncan’s multiple range test level. CON, control; UVB, ultraviolet B; GSH, glutathione; MDA, malondialdehyde; AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Effects of triterpenes (10 μM) on UVB-induced MMP-1 ( A ), MMP-3 ( B ), and collagen ( C ) production in Hs68 cells. Values are means ± SD ( n = 3). Different letters indicate significant ( p < 0.05) differences based on Duncan’s multiple range test level. CON, control; UVB, ultraviolet B; MMP-1, matrix metalloproteinase-1; MMP-3, matrix metalloproteinase-3; AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Control

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Two-dimensional scatter diagram of principal component analysis based on triterpene biological activities. AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside; EA, EA.hy926 cells; HG, HepG2 cells; HS, Hs68 cells; RW, Raw264.7 macrophages; GSH, glutathione; ROS, reactive oxygen species; NO, nitric oxide; TNF-a, tumor necrosis factor-α; C, collagen; AST, aspartate aminotransferase PE, protective effect; IL-6, interleukin-6; MMP3, matrix metalloproteinase-3; ALT, alanine aminotransferase; MDA, malondialdehyde; MMP1, matrix metalloproteinase-1.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Antioxidants

Article Title: Comparison of the Antioxidant Potency of Four Triterpenes of Centella asiatica against Oxidative Stress

doi: 10.3390/antiox13040483

Figure Lengend Snippet: Hierarchical clustering analysis of triterpenes based on triterpene biological activities. AA, asiatic acid; AD, asiaticoside; MA, madecassic acid; MD, madecassoside; EA, EA.hy926 cells; HG, HepG2 cells; HS, Hs68 cells; RW, Raw264.7 macrophages; GSH, glutathione; ROS, reactive oxygen species; NO, nitric oxide; TNF-a, tumor necrosis factor-α; C, collagen; AST, aspartate aminotransferase PE, protective effect; IL-6, interleukin-6; MMP3, matrix metalloproteinase-3; ALT, alanine aminotransferase; MDA, malondialdehyde; MMP1, matrix metalloproteinase-1.

Article Snippet: Hs68 human skin fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Scientific Reports

Article Title: Compatibilizing of cotton fabric with hydrophobic drug cover layer for anti-inflammatory performance with the implementation of ibuprofen

doi: 10.1038/s41598-024-57883-5

Figure Lengend Snippet: The influence of all tested materials on cell viability in MTT reduction assay. The tested materials: raw cotton, A, B, C, D, E, F, G, H, I, J, K and L were added to L929 fibroblasts or human Hs68 fibroblasts cell cultures. The cell viability was estimated as a percentage of cells, which were able to reduce tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT). NC negative control (cells treated with 0.03% H 2 O 2 ), PC positive control (cells in medium alone), raw cotton—tested materials before IBU application. Results are showed as mean ± SD. The black line indicates the minimal percentage of viable cells (70%) required to confirm the biomaterial as non-cytotoxic at the in vitro level. Three experiments were performed in triplicates for each experimental variant. Statistical analysis was performed using the nonparametric U Mann–Whitney test with significance, p < 0.05 (asterisk: unstimulated cells vs. stimulated cells).

Article Snippet: Reference L929 mouse fibroblasts (purchased in LGC Standards, Middlesex, UK) and human Hs68 skin fibroblasts (CRL-1635TM, purchased in American Type Cell Cultures (ATCC), Rockville, MD, USA).

Techniques: MTT Reduction Assay, Negative Control, Positive Control, In Vitro, Variant Assay, MANN-WHITNEY

Journal: Scientific Reports

Article Title: Compatibilizing of cotton fabric with hydrophobic drug cover layer for anti-inflammatory performance with the implementation of ibuprofen

doi: 10.1038/s41598-024-57883-5

Figure Lengend Snippet: Production of pro-inflammatory TNF-a ( A ) and IL-B ( B ) by human Hs68 fibroblasts. The human Hs68 fibroblasts were stimulated with LPS from E. coli alone, as well as with samples B, C, E, F, G and L alone or samples in combination with LPS from E. coli (1 µg/mL) or with IBU alone and with LPS from E. coli in combination with ibuprofen (IBU. 50 µg/mL). Control cells were subcultured in the medium alone. The supernatants were collected for the assessment of interleukin (IL)-1β or IL-TNF-a by the enzyme-linked immunosorbent assay (ELISA). The results are presented as mean values ± SD. Three experiments were conducted in triplicates for each experimental variant. Statistical analysis was performed using the nonparametric U Mann–Whitney test with significance, p < 0.05 (asterisk: unstimulated cells vs. stimulated cells); or Kruskal–Wallis test (filled circle: LPS vs LPS + dressings).

Article Snippet: Reference L929 mouse fibroblasts (purchased in LGC Standards, Middlesex, UK) and human Hs68 skin fibroblasts (CRL-1635TM, purchased in American Type Cell Cultures (ATCC), Rockville, MD, USA).

Techniques: Control, Enzyme-linked Immunosorbent Assay, Variant Assay, MANN-WHITNEY